Fragiskos N. Kolisis Professor in Enzyme Technology
(Responsible for the Laboratory of Biotechnology School of Chemical Engineering National Technical University of Athens)
National Hellenic Research Foundation
Athens, Greece
Tel. +30 210.72.73.759
Tel. +30 210.72.73.765
E-mail: kolisis, kolisis@chemeng.ntua.gr,
Research positions
1973 - 1975 Laboratory of Biochemistry , University of Southampton, UK
1977-1983 Laboratory of Enzymology, Institute of Biological Research and Biotechnology National Hellenic Research Foundation
1984-1985 Enzyme Technology Laboratory, Universite de Technologie de Compiegne, France
1985-1989 Laboratory of Enzymology, Institute of Biological Research and Biotechnology National Hellenic Research Foundation)
1990-to now Biotechnology laboratory, School of Chemical Engineering, NTUA.
2005 - Institute of Biological Research and Biotechnology, National Hellenic Research Foundation
Academic Activities
Member of the Biotechnology Committee of IUPAC (2005 - )
Member of the stirring committee of the Section of Applied Biocatalysis of ôçò European Federation of Biotechnology (2001- )
Member of the stirring committee of The European Federation for Lipids Science and Technology from 2003 -
Member of the general assembly of the European Federation of Biotechnology (EFB)
Member of the Managment Committee COST/EU (1996- until now for the actions D 6, D 10, D 25 )
Member of the Stirring Committee of the School of Chemical Engineering of NTUA (1999-2000 )
Member of the Stirring Committee of the School of Chemical Engineering of NTUA (1999-2000 )
General Secretary of Panhellenic Union of Biotechnology (1988-1991)
Member of the Working Party "Applied Biocatalysis" of EFB (from 1988)
Chairman of the of Panhellenic Union of Biotechnology (1991-94)
Member of the stirring committee of Panhellenic Union of Biotechnology (1994- )
Director of the Division of Synthesis and Development of Industrial processes of the School of Chemical Engineering, NTUA (1999-2000).
Other relevant activities
Referee in international journals (Biotechnology and Bioengineering, Applied Biocatalysis, Journal of Colloid and Interface Science, Biocatalysis and Biotransformation, European Journal of Lipid Ó cience and Technology, J. o f Molecular Catalysis B. Enzymatic, and others)
Member of the Advisory Board of the Biocatalysis and Biotransformation
Member of the Advisory Board of the European Journal of Lipid Science and Technology
Invited speaker, Session Chairman in National and International Conferences
Financial support in research activities
1. 1985-1989 : Âioconversion of hydrophobic and hydrophilic compounds by enzyme systems European Union ( BAP)
2. 1991-1994 : Pharmaceuticals. Application of supercritical gases, microemulsions, organic solvents European Union ( BRIDGE)
3. 1993-1997 Ô: Biocatalysis under extreme or non-classic conditions European Union ( COST/D 6)
4. 1994-1996 : Structure-function lipase relationships in low water media European Union (BIOTECH)
5. 1994-1997 : Production of sugar fatty acid esters from renewable agricultural resources European Union (ÁAIR)
6. 1994-1997 : New strategies for the development of integrated processes for the generation of compounds with flavouring and/or other functional properties from natural material European Union ( AAIR)
7. 1994-1997 : Reactivite des esters gras et du glycerol/ nouveaux procedes. European Union (AAIR)
8. 1998-2002 : Enzymes as Asymmetric Catalysts in Transformations of Organic Molecules and Bio-molecules: Application to Compounds of Practical Importance. European Union (COST/D 10)
9. 1998-2000 : Enzymatic modification of natural antioxidants. Greek General Secretariat for Research and Technology
10. 1999-2001 : Enzymatic improvement of mastic oil biological propertiesproperties. Greek General Secretariat for Research and Technology
11. 1998-2003 : Catalysis of sequence selective amide and peptide cleavage by antibodies and proteins. European Union ( TMR)
12. 2003-2005 : Enzymic Processes - Tools for the Synthesis of Environmentally Friendly Products Greek General Secretariat for Research and Technology . Greek - Czech collaboration program.
13. 2005-2007 : Enzymatic modification of mastic oil constituents for the improvement of its biological activity in relation to angiogenesis.
14. 2005 - 2007 : Biotechnological production of Bioethanol from cellulose derivaties. Greek General Secretariat for Research and Technology
International Collaborations
Universities
Enzyme Technology Laboratory, Universite de Technologie de Compiegne, France
Protein and Cellular Engineering Lab., Universite de la Rochelle, France
Chemical Engineering Department, Technological University of Hannover, Germany.
Department of Biochemistry, University of Napoli, Italy. 6. C.S.I.C.,
Institute of Catalysis, Autonoma Universidade Madrid, Spain.
Institut National de Science Applique, Toulouse, France.
Enzyme Biotechnology, University of Munster, Germany
CNRS, Marseille, France.
SINEF, University of Trodheim, Norway.
Institut National Polytechnique de Loraine, Nancy, France.
Biochemical Engineering Department, Technical University of Lisbon, Portugal.
Institut National Polytechnique Toulouse, France.
Enzyme Technology Laboratory, Strathelyde, U.K.
Instituto Technologica, Universidad Polytechnica, Valencia, Spain.
Industries
Merck, Germany
Great Lakes Chemical, Konstands, Germany
Unilever, The Netherlands
Recordatti Chemicals, Milan, Italy
Organisation Nationale Interprofessionelle des Oleagineux (ONIDOL), France
Institut Francais du Petrol, France
Institut des Corps Gras (ITERG), France
CECA SA, Levallois Perret, France
Oxford Asymmetry LTD, Abingdon, UK
Chiroscience LTD, Cambridge, UK
ÂÉÏÑÕË
Publications
over 100
Participation in Conferences 30 National
100 International
Recent Relevant Publications (2003 - 2005)
Franqueville, Å ., Loutrari, H., Mellou, F., Stamatis, H., Friboulet, A. and Kolisis F.N.). Reverse micelles, a system for antibody-catalysed reactions. J. Mol. Catalysis B: Enzymatic 15-20, 2003
.A. Kontogianni, V. Skouridou, V. Sereti, H. Stamatis and F.N. Kolisis. Lipase-catalysed esterification of rutin and naringin with fatty acids of medium carbon chain. J. Mol. Catalysis B: Enzymatic, 59-62, 2003
V. Skouridou, H. Stamatis, F.N. Kolisis. Lipase-mediated epoxidation of á -pinene. . J. Mol. Catalysis B: Enzymatic 67 -69, 2003
V. Skouridou, H. Stamatis and F.N. Kolisis. Use of essential oils as media for the enantioselective esterification of the monoterpene perillyl alcohol catalysed by lipase. European Journal of Lipid Science and Technology 2003, 328-334..
Vasso Skouridou, Haralambos Stamatis Fragiskos N. Kolisis A study on the process of the lipase-catalysed synthesis of á - pinene oxide in organic solvents, Biocatalysis and Biotransformation, 112-120.
A. Chatziioannou, G. Palaiologos. F.N. Kolisis. Metabolic Flux Analysis as a Tool for the Elucidation of the Mechanism of the Metabolism of Neurotransmitter Glutamate. J. Metabolic Engineering (2003), 201-210.
V. Skouridou, E. D. Chrysina, H. Stamatis , N. G. Oikonomakos, F. N. Kolisis. Kinetic and modeling studies on the lipase catalysed enantioselective esterification of (+-) perillyl alcohol. J. Mol. Catalysis B: Enzymatic 2004 (29), 9-12.
A. K. Tsakalof, K.A. Bairachtari, I.S. Aslani, I.D. Chryssoulakis, F.N. Kolisis. Impact of biological factor on binding media identification in art objects: Identification of animal glue in the presence of Aspergillus niger.J of Sep. Sci. (2004), 27, 1-7.
H. Loutrari, M. Hatziapostolou, V. Skouridou, E. Papadimitriou, C. Roussos, F.N. Kolisis A. Papapetropoulos (2004) J. Pharmacology Experimental Therapeutics, 311 (2), 568-575.
Z. Wimmer, V. Skouridou, M. Zarevuckta, D. Sama, F.N. Kolisis . Enzymic resolution of 2-substituted cycloexanols though lipase-mediated esterification. Tetrahedron Assymetry (2004) 15, 3911 - 3917.
F. Mellou, D. Lazari, H. Skaltsa, A. Tselepis, F. Kolisis, H. Stamatis. Biocatalytic preparation of acylated derivatives of flavonoid glycosides enchances their antioxidant and antimicrobial activity. J. Biotechnology (2005) 0-10.
A. Chatziioannou, G. Palaiologos, F. N. Kolisis, Elucidating the regulatory role of glucocorticoids in the release of neurotransmitter glutamate with the use of metabolic
engineering methodologies. Systems Biology, (under revision)
Professor in Enzyme Technology 
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