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Curriculum Vitae

Vassilis L. Souliotis, Research Associate Professor

ADDRESS
National Hellenic Research Foundation,
Institute of Biological Research and Biotechnology,
48, Vas. Constantinou Ave., 11635 Athens, Greece
Tel.: +30.210.727374
e-mail: vls

RESEARCH PROFILE
Dr Vassilis L. Souliotis has been a group leader at the Institute of Biological Research and Biotechnology, National Hellenic Research Foundation since 2001. His research interests focus on the investigation of the role of DNA damage and repair in the mechanism of chemical carcinogenesis as well as the application of molecular methods in the risk assessment from chemical carcinogens. As a model he uses alkylating agents: (1) N-Nitroso-compounds of environmental relevance which are suspected of playing a role in the causation of human cancer, and (2) alkylating cytostatic drugs (e.g. melphalan, cyclophosphamide), in order to assess the prospect of designing cancer chemotherapy protocols of improved efficacy through the optimization of the induction of cytotoxic DNA damage and to examine interindividual variation in the cellular DNA damage response as molecular marker for predicting differences in therapeutic response and long-term outcome. In the past he had conducted extensive similar studies on DNA damage and repair by certain methylating cytostatic drugs (procarbazine, dacarbazine).

BIOGRAPHICAL SKETCH
 
NAME
Vassilis L. Souliotis		 POSITION TITLE
Research Associate Professor, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation
EDUCATION/TRAINING  (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION DEGREE
(if applicable)		 YEAR(s) FIELD OF STUDY
       
University of Athens
University of Patras 		B.Sc.
PhD		 1981
1987		 Chemistry
Molecular Biology
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation   1987-1994 Post-doc, Molecular Biology

 

 

 

 

POSITIONS AND EMPLOYMENT

1981-1985: PhD student, Program of Genetic Engineering, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation
1987-1994: Postdoctoral Fellow, Program of Chemical Carcinogenesis and Genetic Toxicology, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation
1994-2001: Research Assistant Professor, Program of Chemical Carcinogenesis and Genetic Toxicology, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation
2001-present: Research Associate Professor, Program of Chemical Carcinogenesis and Genetic Toxicology, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation

PARTICIPATION IN INTERNATIONAL PROJECTS

- EEC 3rd Environmental Research Programme: "Genetic effects of environmental chemicals- Assay of DNA methylation and study of its mutagenic properties in defined sequencies" (1987-1991)
- International Agency for Research on Cancer: Collaborative Research Agreement "Assay of DNA methylation induced in Hodgkin's lymphoma patients during chemotherapy by procarbazine" (1989-1991)
- EEC BIOMED (EUROGAST NETWORK): "Detection of DNA methylation adducts in peripheral blood leukocytes of populations at different risks of gastric cancer" (1990-1992)EEC STEP Programme: "Dosimetry of genetic effects of environmental chemicals" (1991-1994)
- EEC Toxicology Action Programme:''The Toxicology of Chemicals. Carcinogenicity. Summary reviews of the scientific evidence'' (1992-1993)
- EEC ENVIRONMENT Programme: "Dosimetry and modelling of methylating agent mutagenesis in transgenic mice and biomonitoring of human populations environmentally exposed to methylating agents" (1993-1996)
- EEC BIOMED: ''DNA Repair network'' (1994-1996)
- EEC BIOMED: "Markers of DNA damage and risk of second malignancy following chemotherapy" (1994-1996)
- EEC Quality of life and living resourches, Environment and health key action.''Mechanism-based approaches to improved cancer risk assessment of ambient air polycyclic aromatic hydrocarbons (AMBIPAH project)'' (2002-2006)
- ECNIS: Environmental Cancer, Nutrition and Individual Susceptibility, FP6 NoE (www.ecnis.org) (2005-2010)

PUBLICATIONS IN REFEREED JOURNALS
1. Patrinou-Georgoula,M., Souliotis,V.L. and Dimitriadis,G.J. (1987) A study on the developmental appearance of serum proteins and its mRNA in the insect Dacus oleae. Comp.Biochem.Physiol., 87Â, 79-88.
2. Souliotis,V.L., Patrinou-Georgoula,M., Zongza,V. and Dimitriadis,G.J. (1988) Cuticle proteins during the development of Dacus oleae. Insect Biochem., 18, 485-92.
3. Souliotis,V.L., Patrinou-Georgoula,M., Zongza,V. and Dimitriadis,G.J. (1988) Isolation and characterization of mRNAs coding for the third instar larvae cuticle proteins of Dacus oleae. Insect Biochem., 18, 829-37.
4. Souliotis,V.L. and Dimitriadis,G.J. (1989) Identification and molecular analysis of the cuticle protein genes of Dacus oleae. Insect Biochem., 19, 499-507.
5. Souliotis,V.L., Giannopoulos,A., Koufakis,I., Kaila,S., Dimopoulos,C. and Kyrtopoulos,S.A. (1989) Development and  validation of a new assay for O6-alkylguanine-DNA-alkyltransferase based on the use of an oligonucleotide substrate, and its application to the measurement of DNA repair activity in extracts of biopsy samples of human urinary bladder mucosa. Carcinogenesis, 10, 1203-8.
6. Souliotis,V.L. and Kyrtopoulos,S.A. (1989) A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on  repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. Cancer Res., 49, 6997-7001.
7. Souliotis,V.L., Patrinou-Georgoula,M., Zongza,V. and Dimitriadis,G.J. (1990) The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae. Cell Differ.Dev., 31, 23-9.
8. Souliotis,V.L., Kaila,S., Boussiotis,V.A., Pangalis,G.A. and Êyrtopoulos,S.A. (1990) Accumulation of O6-methylguanine in human blood leukocyte DNA during exposure to procarbazine and  its relationships with dose and repair. Cancer Res., 50, 2759-64.
9. Souliotis,V.L., Boussiotis,V.A., Pangalis,G.A. and Kyrtopoulos,S.A. (1991) In vivo formation and repair of O6-methylguanine in human leukocyte DNA after intravenous exposure to dacarbazine. Carcinogenesis, 12, 285-8.
10. Kyrtopoulos,S.A., Souliotis,V.L., Ambatzi,P., Pangalis,G., Boussiotou,V., Haritopoulos,G. and Davaris,G. (1991) Novel, sensitive assays for O6-alkylguanine and its repair and their application to studies of the molecular epidemiology of this lesion in human populations. IARC Sci. Publ., 105, 78-82.
11. Souliotis,V.L., Zongza,V., Nikolopoulou,V. and Dimitriadis,G.J. (1992) Measurement of O6-methylguanine-type adducts in DNA and O6-alkylguanine-DNA-alkyltransferase repair activity in normal and neoplastic human tissues. Comp.Biochem.Physiol., 101B, 269-75.
12. Kyrtopoulos,S.A., Souliotis,V.L., Valavanis,C., Boussiotis,V.A. and Pangalis,G.A. (1993) Accumulation of O6-methylguanine in human DNA after therapeutic exposure to methylating agents and its relationship with biological effects. Environ.Health Perspect., 99, 143-7.
13. Sfikakis,P.P., Souliotis,V.L. and Panayiotidis,P.P.(1993) Suppression of Interleukin-2 and Interleukin-2 receptor biosynthesis by gold compounds in in vitro activated human peripheral blood mononuclear cells. Arthritis Rheum., 36, 208- 12.
14. Souliotis,V.L., Valavanis,C., Boussiotis,V.A., Pangalis,G.A. and Kyrtopoulos,S.A. (1994) Comparative dosimetry of O6-methylguanine in humans and rodents treated with procarbazine. Carcinogenesis, 15, 1675-80.
15. Valavanis,C., Souliotis,V.L. and Kyrtopoulos,S.A. (1994)  Differential effects of procarbazine and methylnitrosourea on the accumulation of O6-methylguanine and the depletion and recovery of O6-alkylguanine-DNA alkyltransferase in rat tissues. Carcinogenesis, 15, 1681-8.
16. Pletsa,V., Troungos,C., Souliotis,V.L. and Kyrtopoulos,S.A. (1994) Comparative study of mutagenesis by O6-methylguanine in the human Ha-ras oncogene in E.coli and in vitro. Nucleic Acids Res., 22, 3846-53.
17. Theocharis,S.E., Souliotis,V.L., Panayiotidis,P.G. (1994). Suppression of interleukin-1â and tumor necrosis factor-á biosynthesis by cadmium in in vitro activated human peripheral blood mononuclear cells. Arch.Toxicol., 69, 132-6.
18. Anderson,L.M., Chhabra,S.K., Nerurkar,P.V., Souliotis,V.L. and Kyrtopoulos,S.A. (1995) Alcohol-related cancer risk: a toxicokinetic hypothesis. Alcohol, 12, 97-104.
19. Souliotis,V.L., Chhabra,S.K., Anderson,L.M. and Kyrtopoulos,S.A. (1995) Dosimetry of O6-methylguanine in rat DNA after low-dose, chronic exposure to N-nitrosodimethylamine (NDMA). Implications for the mechanism of NDMA hepatocarcinogenesis. Carcinogenesis, 16, 2381-7.
20. Chhabra,S.K., Souliotis,V.L., Harbaugh,J., Krasnow,S.W., Jones,A.B., Anderson,L.M. and Kyrtopoulos,S.A. (1995) O6-Methylguanine DNA adduct formation and modulation by ethanol in placenta and fetal tissues after exposure of pregnant patas monkeys to N-nitrosodimethylamine. Cancer Res., 55, 6017-20.
21. Philip,P.A., Souliotis,V.L., Harris,A.L., Salisbury,A., Tates,A.D., Mitchell,K., van Delft,J.H.M., Ganesan,T.S., and Kyrtopoulos,S.A. (1996) Methyl DNA adducts, DNA repair and Hypoxanthine-guanine phosphoribosyl transferase mutations in peripheral white blood cells from patients with malignant melanoma treated with dacarbazine  and hydroxyurea. Clin.Cancer Res., 2, 303-10.
22. Sfikakis,P.P., Souliotis,V.L., Katsilambros,N., Markakis,K., Vaiopoulos,G., Tsokos,G.C. and Panayiotidis, P. (1996) Down-regulation of Interleukin-2 and á-chain Interleukin-2 receptor biosynthesis by  cis-platin in human peripheral lymphocytes. Clin.Immunol.Immunopathol., 79, 43-9.
23. Anderson,L.M., Souliotis,V.L., Chhabra,S.K., Moskal,Ô.J., Harbaugh,S.D. and Kyrtopoulos,S.A. (1996) N-Nitrosodimethylamine-derived O6-methylguanine in DNA of monkey gastrointestinal and urogenital organs and enhancement by ethanol. Int.J.Cancer, 66, 130-4.
24. Souliotis,V.L., Valavanis,C., Boussiotis,V.A., Pangalis,G.A. and Kyrtopoulos,S.A. (1996) Comparative study of the formation and repair of O6-methylguanine in humans and rodents treated with dacarbazine. Carcinogenesis, 17, 725-32.
25. van Delft,J.H.M., Luiten-Schuite,A., Souliotis,V.L., Kyrtopoulos,S.A., Ouwerkerk,J., Keizer,H.J. and Baan,R.A. (1996) N7-Methylguanine and O6-methylguanine levels in DNA of white blood cells from cancer patients treated with dacarbazine. Biomarkers, 1, 94-8.
26. Chhabra,S.K., Souliotis,V.L., Kyrtopoulos,S.A. and Anderson,L.M. (1996) Nitrosamines, alcohol and gastrointestinal tract cancer: Recent epidemiology and experimentation. In Vivo, 10, 265-84.
27. Bianchini,F., Weiderpass,E., Kyrtopoulos,S.A., Souliotis,V.L., Henry-Amar,M., Wild,C.P. and Boffetta,P. (1996) Detection of DNA methylation adducts in Hodgkin's disease patients treated with procarbazine. Biomarkers, 1, 226-31.
28. Farmer,P.B., Sepai,O., Lawrence,R., et al. (1996) Biomonitoring human exposure to environmental carcinogenic chemicals. Mutagenesis, 11, 363-81.
29. Kyrtopoulos,S.A., Souliotis,V.L., Chhabra,S.K. and Anderson,L.M. (1996) DNA damage studies related to the assessment of the role of N-nitroso compounds in human cancer. European Journal of Cancer Prevention, 5, 109-14.
30. Sfikakis,P.P., Souliotis,V.L., Akbar,A.N., Katsilambros,N., Hoffbrand,A.V. and Panayiotidis,P. (1996)  Regulation of bcl-2 and fas expression in primary activation of human peripheral lymphocytes is not sensitive to dexamethasone or cyclosporin-A. Hum.Immunol., 50, 121-6.
31. Kyrtopoulos,S.A., Anderson,L.M., Chhabra,S.K., Souliotis,V.L, Pletsa,V., Valavanis,C. and Georgiadis,P. (1997) DNA adducts and the mechanism of carcinogenesis and cytotoxicity of methylating agents of environmental and clinical significance. Cancer Detect.Prev., 21, 391-405.
32. Saunders,M.P., Salisbury,A.J., O' Byrne,K.J., Souliotis,V.L., Varcoe,S.M., Talbot,D.C.,  Kyrtopoulos,S.A. and Harris,A.L. (1997) A phase II study evaluating the effect of tamoxifen on DNA repair in melanoma patients treated with dacarbazine. Anticancer Res., 17, 4677-80.
33. Webb, P.M., Bates,C.J., Palli,D., Forman,D. and The EUROGAST Study Group (1997) Gastric cancer, gastritis and plasma vitamin C: Results from  an international correlation and cross-sectional study. Int.J.Cancer, 73, 684-9.
34. Souliotis,V.L., van Delft,J.H.M., Steenwinkel,M.J., Baan,R.A.  and Kyrtopoulos,S.A. (1998) DNA adducts, mutant frequencies and mutation spectra of ëlacZ transgenic mice treated with N-nitrosodimethylamine. Carcinogenesis, 19, 731-9.
35. Sfikakis,P.P., Dimopoulos,M.A., Souliotis,V.L., Charalambopoulos,D., Mavrikakis,M. and Panayiotidis,P. (1998) Effects of 2-chlorodeoxyadenosine and gold sodium  thiomalate on human bcl-2 gene expression. Immunopharmacology and Immunotoxicology, 20, 63-78.
36. Chhabra,S.K., Anderson,L.M., Perella,C., Desai,D., Amin,S., Kyrtopoulos,S.A. and Souliotis,V.L. (2000) Coexposure to ethanol with N-nitrosodimethylamine or 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone during lactation of rats: marked increase in O6-methylguanine-DNA adducts in maternal mammary gland and in suckling lung and kidney. Toxicol.Appl.Pharmacol., 169, 191-200.
37. Souliotis V.L., Henneman J.R., Reed C.D., Chhabra S.K., Diwan B.A., Anderson L.M., Kyrtopoulos S.A. (2002) DNA adducts and liver DNA replication in rats during chronic exposure to N-nitrosodimethylamine (NDMA) and their relationships to the dose-dependence of NDMA hepatocarcinogenesis. Mutat.Res., 500, 75-87.
38. Katsiari C.G., Liossis S.N., Souliotis V.L., Dimopoulos A.M., Manoussakis M.N., Sfikakis P.P. (2002) Aberrant expression of the costimulatory molecule CD40 ligand on monocytes from patients with systemic lupus erythematosus. Clin.Immunol., 103, 54-62.
39. Souliotis V.L., Dimopoulos M.A., Sfikakis P.P. (2003) Gene-specific formation and repair of DNA monoadducts and interstrand cross-links after therapeutic exposure to nitrogen mustards. Clin.Cancer Res., 9, 4465-74.
40. Souliotis V.L., Sfikakis P.P., Anderson L.M., Kyrtopoulos S.A. (2004) Intra- and intercellular variations in the repair efficiency of O6-methylguanine, and their contribution to kinetic complexity. Mutat.Res., 568, 155-170.
41. Dimopoulos M.A., Souliotis V.L., Anagnostopoulos A., Papadimitriou C., Sfikakis P.P. (2005) Extent of damage and repair in the p53 tumor-suppressor gene after treatment of myeloma patients with high-dose melphalan and autologous blood stem-cell transplantation is individualized and may predict clinical outcome. J.Clin.Oncol., 23, 4381-9.
42. Singh R., Gaskell M., Le Pla R.C., Kaur B., Azim-Araghi A., Roach J., Koukouves G., Souliotis V.L., Kyrtopoulos S.A., Farmer P.B. (2006) Detection and quantitation of benzo[a]pyrene-derived DNA adducts in mouse liver by liquid chromatography-tandem mass spectrometry: comparison with 32P-postlabeling. Chem.Res.Toxicol., 19, 868-78.
43. Souliotis V.L., Dimopoulos M.A., Episkopou H.G., Kyrtopoulos S.A., Sfikakis P.P. (2006) Preferential in vivo DNA repair of melphalan-induced damage in human genes is greatly affected by the local chromatin structure. DNA Repair, 5, 972-85.
44. Sfikakis P.P., Souliotis V.L., Fragiadaki K., Moutsopoulos H., Boletis J., Theofilopoulos A. (2007) Increased Expression of the FoxP3 functional marker of Regulatory T cells following B Cell Depletion with Rituximab in patients with Lupus Nephritis. Clinical Immunol., 123, 66-73.
45. Dimopoulos M.A., Souliotis V.L., Anagnostopoulos A. et al. (2007) Melphalan-induced DNA damage in vitro as a predictor for clinical outcome in multiple myeloma. Haematologica/The Hematology Journal, 92, 1505-12.
46. Episkopou H., Kyrtopoulos S.A., Sfikakis P.P., Fousteri M., Dimopoulos M.A., Mullenders L.H.F., Souliotis V.L. (2009) Association between transcriptional activity, local chromatin structure and the efficiencies of both subpathways of nucleotide excision repair of melphalan adducts. Cancer Res., 69, 4424-33.

CHAPTER IN COLLECTIVE VOLUMES
Souliotis V.L., Kyrtopoulos S.A. Dose-response relationships and potential thresholds in the induction of mutagenesis and the influence of DNA repair and cell cycle progression, in Dietrich C., Oesch F., Oesch-Bartlomowicz B., Waiss C. (eds): Mechanisms of chemical carcinogenesis and their impact on dose-response relationships-the examples of dioxin and benzo(a) pyrene. ECNIS Review, 2008, pp 31-42.

TOTAL NUMBER OF CITATIONS: ~590
SUM JOURNAL IMPACT FACTOR: ~185
 
   

Last Update: 29.10.2010
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