Aris Xenakis, Researcher Director (Program Head) Dr. Vassiliki Papadimitriou, Researcher C', Chemist
PhD Students Mrs. Argyro Kalaitzaki, Chemical Engineer, University of Orebro, Sweden Mrs. Karen Goncalves, Pharmacist, Federal University of Rio de Janeiro, Brazil Mr. Ivaldo Junior, Pharmacist, Federal University of Rio de Janeiro, Brazil Ms. Maria Hatzidaki, Chemical Engineer, University of Orebro, Sweden Ms. Andreia Fatima Zanette, Chemical Engineer, University of Marenga, Brazil
Msc. Students Ms. Tina Filippou, Biologist Ms. Ioanna Zampakidi, Biologist
Technicians Mr. G. Sotiroudis, MSc. Pharmacist
Aims of the Programme
Model of Reverse Micelle
The Biomimetics and Nanobiotechnology Programme deals with studies on the behavior of biomolecules, such as enzymes, in model systems simulating their natural environment. The studies are focused on structural and functional aspects of the biomolecules when hosted in nano-scale environments with restricted water contents, such as nanodispersions of the microemulsion type.
In these systems the coexistence of water nanodroplets in a continuous non-polar organic phase (oil) allows the solubilisation of substances with various polarities, such as lipophilic ones localized in the continuous oil phase, or hydrophilic ones that are encapsulated in the dispersed aqueous phase and that may react through a huge interface. It is possible, thus, to incorporate enzymes and use them as efficient biocatalysts of lipophilic substrates.
Ενσωμάτωση ενζύμου σε αντίστροφα μικκύλια. Πιθανές θέσεις εντοπισμού του ενζύμου
The activities developed within the Biomimetics and Nanobiotechnology programme comprise:
AOT in the absence of lipase AOT in the presence of lipase
lecithin in the absence of lipase lecithin in the presence of lipase
These studies have yielded various applications, which are developed within the Industrial Enzymology Unit of the Division of Biological Research and Biotechnology, in collaboration with Food, Pharmaceutics, and Cosmetics industries.
Current Externally Funded projects:
Current Externally Funded projects:
MED Programme 2009-2011 "Mediterranean Transnational Technology Transfer - MET3" Budget: 360.000 €
South East Europe - Trasnational Cooperation Programme 2009-2011, "Solutions and Interventions for the Technological Transfer and the Innovation of the Agro-Food Sector in the South East Regions", SEE/A/160/1.1/X Budget: 238.118 €
MED Programme 2010-2012 «IP Awareness and Enforcement: innovative services for Mediterranean SMEs -IP-SMEs» No. 1G-MED08-309 (2010-2) Budget: 170.000 €
Programme ΕΣΠΑ "Cooperation" 2010-2. "Development of nanoemulsions as new materials for managing phytoprotective products to reduce the environmental charge" Budget: 449.040,00 € (NHRF 147.743€). Coordinator Dr V. Papadimitriou.
E.D. Tzika, M. Christoforou, S. Pispas, M. Zervou, V. Papadimitriou, T. G. Sotiroudis, E. Leontidis, A. Xenakis (2011) "Influence of nanoreactor environment and substrate location on the activity of horseradish peroxidase in olive oil-based w/o microemulsions" Langmuir, 27, 2692-2700.
V. Papadimitriou, E. D.Tzika, S. Pispas, T.G. Sotiroudis, A. Xenakis (2011) "Microemulsions based on virgin olive oil: a model biomimetic system for studying native oxidative enzymatic activities" Colloids Surfaces.A: Physichochemical & Engineerinig Aspects, 382, 232-237
M. Fanun, V. Papadimitriou, A. Xenakis (2011) "Characterization of cephalexin loaded nonionic microemulsions" J. Colloid Interf. Sci., 361, 115-21
A. Xenakis, V. Papadimitriou, T.G. Sotiroudis (2010) "Colloidal structures in natural oils" Curr.Opinion Colloid Interface Sci. 15, 55-60
M. Zoumpanioti, H. Stamatis, A. Xenakis (2010) "Microemulsion-based organogels as matrices for lipase immobilization" Biotechnol.Advances, 28, 395-406
F. Michaux, M. Zoumpanioti, M. Papamentzelopoulou, M. J. Stebe., J. L. Blin, A. Xenakis (2010) "Immobilization and activity of Rhizomucor miehei lipase. Effect of the matrix properties prepared from nonionic fluorinated surfactants". Proc.Biochem. 45, 39-46
A. Xenakis, V. Papadimitriou, H. Stamatis, F. N. Kolisis (2009) "Biocatalysis in microemulsuions" in "Microemulsions: Properties and Applications" Surfactant Sci.Ser., Vol.144, pp.349-385. Ed. M.Fanun.,CRC Press, Jerusalem, Israel
V. Papadimitriou, S. Pispas, S. Syriou, A.Pournara, M. Zoumpanioti, T. G. Sotiroudis, A.Xenakis (2008) "Biocompatible Microemulsions based on Limonene: Formulation, Structure and Applications" Lamgmuir, 24, 3380-3386.
V. Papadimitriou, T.G. Sotiroudis, A. Xenakis, (2007) Olive oil microemulsions: enzymatic activities and structural characteristics, Langmuir, 23, 2071-7.
S. Avramiotis, V. Papadimitriou, E. Hatzara, V. Bekiari, P. Lianos, A. Xenakis (2007) "Lecithin Organogels Used as Bioactive Compounds Carriers. A Microdomain Properties Investigation" Langmuir, 23, 4438-4447