PhD Students Ms. Maria Hatzidaki, Chemical Engineer, University of Orebro, Sweden
Msc. Students Leandros Sklaviadis, Food Scientist Ioanna Theohari, Chemist
Model of Reverse Micelle
The Biomimetics and Nanobiotechnology group deals with studies on the behavior of biomolecules, such as enzymes, in model systems simulating their natural environment. The studies are focused on structural and functional aspects of the biomolecules when hosted in nano-scale environments with restricted water contents, such as nanodispersions of the microemulsion type.
In these systems the coexistence of water nanodroplets in a continuous non-polar organic phase (oil) allows the solubilisation of substances with various polarities, such as lipophilic ones localized in the continuous oil phase, or hydrophilic ones that are encapsulated in the dispersed aqueous phase and that may react through a huge interface. It is possible, thus, to incorporate enzymes and use them as efficient biocatalysts of lipophilic substrates.
The activities developed within the Biomimetics and Nanobiotechnology group comprise:
These studies have yielded various applications, which are developed within the Industrial Enzymology Unit of the Institute of Biological Research and Biotechnology, in collaboration with Food, Pharmaceutics, and Cosmetics industries.
Schematic representation of microemulsions
AOT in the absence of lipase AOT in the presence of lipase
lecithin in the absence of lipase lecithin in the presence of lipase
These studies have yielded various applications, which are developed within the Industrial Enzymology Unit of the Division of Biological Research and Biotechnology, in collaboration with Food, Pharmaceutics, and Cosmetics industries.
Current Externally Funded projects:
Program Agence Nationale de Recherche (ANR), Institut Carnot, Projet Lipides pour l’industrie et la santé (LISA), France. 2013-14. «micro/nano-émulsions de type e/h biocompatibles et riches en micronutriments : préparation et caractérisation» Budget: 43.000 €. Coordinator: F.Calderon
EU INCO FP7: Mediterranean Science, Policy, Research & Innovation Gateway (MEDSPRING), , 2013-6 Budget: 289.650 €. Coordinator NHRF: Dr G.Bonas & A. Xenakis
EU INCO FP7: EURO-MEDITERRANEAN Cooperation through ERANET joint activities and beyond (ERANETMED, 2013-6, Budget: 107.649,45 €. Coordinator NHRF: Dr G.Bonas & A. Xenakis
Program National Strategic Reference Framework, Action “Developmental Projects of Research Organisations- Kripis”2013-2015:“STHENOS”, Targeted therapeutic approaches against degenerative diseases, with special focus on cancer and ageing. Budget: 1.500.000,00 € (Biomimetics 174.773€). Coordinator Dr A. Pintzas
Program ΕΣΠΑ PAVET “Application of Nanoemulsion Technology to food products based on Oil in Water Emulsions such as vegetable cream and fudge icings” Budget: 300.000,00 € Contractor: JOTIS SA (NHRF 61.250€).
Kalaitzaki, A. Xenakis, V. Papadimitriou “Highly Water Dilutable Microemulsions: A structural study” Colloid Polym.Sci. (2015) DOI 10.1007/s00396-014-3496-1.
N. Tsinisizeli, G. T. Sotiroudis, A. Xenakis, K.E. Lykeridou “Determination of nicotine and cotinine in meconium from Greek neonates and correlation with birth weight and gestational age at birth” Chemosphere (2015) 119, 1200-7. DOI:10.1016/j.chemosphere.2014.09.094.
V. Sereti, M. Zoumpanioti, V. Papadimitriou, S. Pispas, A. Xenakis “Biocolloids Based on Amphiphilic Block Copolymers as a Medium for Enzyme Immobilization” J.Phys.Chem. B (2014) 118, 9808 – 9816. DOI: 10.1021/jp504449y. (IF2013 3.377).
Itabaiana-Jr, K.M. Gonçalves, M. Zoumpanioti, I.C.R. Leal, L.S.M. e Miranda, A. Xenakis, and R.O.M.A de Souza “Microemulsions-Based Organogels as an Efficient Support for Lipase Catalyzed Reactions under Continuous Flow Conditions”ACS Org.Proc.Res.Dev. (2014) Accepted. DOI: 10.1021/op500136c DOI: 10.1021/op500136c.
A.F. Zanette, I. Zampakidi, G.T. Sotiroudis, M. Zoumpanioti, I.C.R. Leal, R.O.M.A. de Souza, L. Cardozo-Filho, A. Xenakis “Chemo-enzymatic epoxidation catalyzed by C. antarctica lipase immobilized in microemulsion-based organogels” J.Mol.Catal.B. Enzymatic, (2014) 107, 89-94. doi:10.1016/j.molcatb.2014.05.013.
D. Amadei, M.D. Chatzidaki, J. Devienne, J. Monteil, M. Cansell, A. Xenakis, F. Leal-Calderon “Low shear-rate process to obtain transparent W/O fine emulsions as functional foods” Food Res.Intern. (2014) 62, 533-540. doi:10.1016/j.foodres.2014.03.069.
Kalaitzaki, M. Poulopoulou, V. Papadimitriou, A. Xenakis, “Surfactant-rich biocompatible microemulsions for transdermal administration of methylxanthine drugs” Colloids Surfaces.A: Physichochem. Eng.Aspects, (2014) 442, 80-87 doi:10.1016/j.colsurfa.2013.05.055
Kalaitzaki, M. Emo, M.J. Stébé, A. Xenakis, V. Papadimitriou, “Biocompatible nanodispersions as delivery systems of food additives: A structural study” Food Res. Intern. (2013) 54, 1448-1454, doi:10.1016/j.foodres.2013.08. 010.
I.Itabaiana-Jr, K.M.Gonçalves, Y.M.L.Cordeiro, M.Zoumpanioti, I.C.R.Leal, L.S.M.e Miranda, R.M.A Souza , A. Xenakis “Kinetics and mechanism of lipase catalyzed monoacylglycerols synthesis” J.Mol.Catal.B. Enzymatic (2013) 96, 34-39. doi:10.1016/j.molcatb.2013.06.008
Kyriazi, V. Papadimitriou, T. G. Sotiroudis, A. Xenakis “Development and characterization of a digestion model based on olive oil microemulsions” Eur.J.Lipid Sci.Technol., 115, (2013) 601-611
V. Papadimitriou, M. Dulle, W. Wachter, T.G. Sotiroudis, O. Glatter, A. Xenakis “Structure and dynamics of veiled virgin olive oil: Influence of production conditions and relation to its antioxidant capacity” Food Biophys. (2013) 8, 112-121
E.D. Tzika, M. Christoforou, S. Pispas, M. Zervou, V. Papadimitriou, T. G. Sotiroudis, E. Leontidis, A. Xenakis (2011) “Influence of nanoreactor environment and substrate location on the activity of horse-radish peroxidase in olive oil-based w/o microemulsions” Langmuir, 27, 2692–2700.