MEMBRANES

In the present study, protein engineering and directed evolution techniques were applied to identify mutations in the RraA protein sequence that could render it a more effective helper factor for the overexpression of recombinant membrane proteins compared to the wild-type protein. The working hypothesis was that specific mutations in the RraA sequence could enhance its efficacy as a helper factor in the overexpression of recombinant membrane proteins relative to the wild-type (WT) protein. The aim of the intervention was to enhance the researchers’ academic CVs and research skills, in order to improve their prospects for an academic and research career.