Infrastructure

Fully equipped laboratories for the synthesis, isolation, and characterization of bioactive compounds, including:

• Microwave reactor|CEM Discover
• Ultrasound reactor |Sonics & Materials
• Parallel synthesis apparatus |Buchi Syncore
• Large-scale reactor (5L) |Radleys
• High-pressure reactor |Parr 2
• Flow hydrogenation system |Thales Nano H-Cube® Mini PLUS
• Polarimeter |Kruss Optronic P300
• FT-IR spectrometer with ATR unit |Bruker-Tensor 27
• Analytical HPLC |Thermo Fisher Scientific

ΙCB maintains a proprietary compound library with bioactive compounds synthesized by ICB researchers suitable for screening campaigns against various biological targets.

• Varian VRMS 600 MHz with H/C/N cryoprobe (He-cooled)
• Varian VRMS 300 MHz with H/F/C/P probe

The facility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with a new spectrometer:

• Bruker Avance NEO 700 MHz with H& F/C/N TCI PRODIGY cryoprobe and refrigerated sample changer (operation in 2026)

• LTQ Orbitrap Velos UHPLC-MSn |Thermo Fisher Scientific
• LCQ Fleet Ion Trap LC-MSn |Thermo Fisher Scientific

The facility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with the following equipment: (operation in 2026):

• timsMetabo™ MS |Bruker (operation in 2026)
• microFLEX LRF MALDI-TOF-MS |Bruker (operation in 2026)
• Agilent 7850 ICP-MS |Agilent (operation in 2026)

• Robotic Crystallisation, Rock Imager 182®, Formulatrix automated Crystal Imaging with remote access to local and external users (Crystal Hotel)
• X-ray data collection on a Microfocus X-ray diffractometer SuperNova, Agilent

Equipment for protein sample preparation and analysis, including:

• PCRs for gene cloning
• Temperature controlled large capacity shaker incubators
• FPLC- AKTA Purifiers
• Multi-mode microplate readers for enzymatic and fluorescence characterization of protein-ligand interactions
• Speed vacs, high speed refrigerated floor centrifuge and ultracentrifuge

• ΙνLeica 626 TCS SPE (confocal laser scanning microscope) with LAS AF software
• Fluorosescence microscope NIKON with colour digital camera

The facility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with the following equipment:

• ZEISS LSM 980 confocal microscopy system with Airyscan 2 for 4D experiments with ultra-high resolution (operation in 2025)
• System for in vivo fluorescence imaging of small animals (Φ-eye™ system, BIOEMTECH) (operation in 2025)
• System for photoacoustic tomography and ultrasound Imaging (Vevo F2, FUJIFILM VisualSonics) (operation in 2025)

The facility includes:

• NGS- Ion OneTouch 2 System, Tape Station system, Qubit 4 Fluorometer
• Pyrosequencer, Real Time-PCR machines, cloning, electrophoresis and SSCP

The facitlity will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with:

• System for next generation massive parallel sequencing

Ion GeneStudio S5 Prime System |Thermo Fisher Scientific (operation in 2026)

Fully-equipped facilities for cell, tissue and nematode C. elegans cultures including:

• CO₂ incubators
• Constant temperature incubators (15⁰ C, 20⁰ C, 25⁰ C)
• Inverted microscopes
• Stereomicroscopes and fluorescence stereomicroscopes
• Biosafety cabinets
• Automatic cell counter
• Centrifuges and ultra-low temperature freezers

The fascility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with:

• BioTek Cytation C10, Agilent high-resolution confocal microscopy and multimode microplate reader with an automated microplate incubation device (BioTek BioSpa 8). (operation in 2025)

• Flow cytometry analyser (Partec-CyFlow ML)
• High speed multiparameter Cell Sorter BD FACS ARIA^TM II with 2 lasers (488, 633 nm).

The facility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with:

• An additional 375 nm laser source for BD FACS ARIA II and a liquid delivery pump in the main unit for cell isolation under sterile conditions. (operation in 2025)

• Electron Paramagnetic Resonance (EPR) spectrometer (Bruker EMX Micro)
• Dynamic Light Scattering analyzer (DLS) (Zetasizer Nano ZS, Malvern) with capability to measure hydrodynamic diameter, polydispersity index, and zeta potential
• Franz Diffusion Cell
• Liposome Extruder (Mini Extruder with Temperature Control, Avanti Polar Lipids)
• High pressure homogenizer (Panda Plus 1000, GEA Niro Soavi);
• Viscometer (Brookfield)

The facility will be upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with

• Small angle X-ray scattering array Xeuss 3.00 (Xenocs) (operation in 2026)

The Animal House Facility complies with national and international standards (ISO 9001:2015) and provides housing for mice and rats in specialized individually ventilated cage units (IVCs).

The Facility breeds or supplies laboratory animals for cutting-edge technology development, toxicity studies, preclinical research, and educational purposes.

The plant facilities are licensed by the Veterinary Service of the Ministry of Rural Development and Food (EL 25 BIObr 031 – breeding facility; EL 25 BIOsup 032 – supply facility; EL 25 BIO 033exp – research facility).

The facility spans 150 m² with 8 spaces and an antechamber, including quarantine area, furnace, cleaning, model construction, storage, operating room, and office. The air conditioning and environmental control system is fully automated, with remote and cloud-based operation, while the facility is monitored in real time.

The facility has been upgraded within the framework of National Recovery and Resilience Plan “Greece 2.0” and offers small animal imaging capabilities through:

• Photoacoustic Tomography and Ultrasound Imaging (Vevo F2 system, FUJIFILM VisualSonics) (operation in 2025)
• Fluorescence (Φ-eye™ system, BIOEMTECH).

• Μultiprocessor Workstations (equipped with CPU and GPU)
• Cloud-based infrastructure across Google Cloud Platform, Amazon Web Services, and public resources is leveraged.

The computing power of the infrastructure is being upgraded through access to the new High-Performance Computing facility of NHRF (816 cores, 2 GPU accelerators with 96 GB memory and 20,000 CUDA cores, system RAM: 3.5 TB, total storage capacity: ~1 PB) (funded under the National Recovery and Resilience Plan ‘Greece 2.0’, operation in 2025).

Fully equipped laboratories for synthesis, isolation, purification, fractionation, and characterization of conjugated polymers/biomaterials:

• Microwave/ultrasonic reactor
• Shimadzu size exclusion chromatography (SEC) with fractionation capability
• BÜCHI automated column chromatography
• PerkinElmer LS45 fluorescence spectrometers
• VersaSTAT4A potentiostat-galvanostat
• Jeol 7610-F field emission scanning electron microscope (FESEM/EDX)
• Renishaw micro-Raman

The facility will be upgraded within the framework of National Recovery and Resilience Plan “Greece 2.0” with the following equipment:

• JASCO V-770 TA DMA 850 ultraviolet-visible-NIR (UV-Vis-NIR) spectrophotometer with humidity chamber (operation in 2026)
• Inert atmosphere boxes with MBraun thermal evaporator and Enlitech external quantum efficiency (EQE), and Enlitech solar radiation simulator
• Fluxim Paios all-in-one (operation in 2026)
• RAMAN spectrophotometer with AFM/SNOM, WITec alpha300R (operation in 2026)
• Small angle X-ray scattering array Xeuss 3.00 (Xenocs) (operation in 2026)
• Electron Microscopes Krios G4, Spectra 200, Aquilos 2, Talos 120 |Thermo Fisher Scientific (operation in 2026)