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CLOSENewly acquired equipment at NHRF
ICB together with the TPCI, operate a large set of advanced instrumentation, acquired through funding from the European Union – NextGenerationEU, co-financed by national resources and the European Investment Bank, within the framework of the National Recovery and Resilience Plan ‘Greece 2.0’.
The state-of-the-art equipment comprises among others, a full electron microscopy suite (cryo-EM, HR-TEM, ΤΕΜ, cryo-FIB-SEM), AFM-Raman, SAXS/WAXS, XRD, MALDI-TOF-MS, timsMetabo™ MS, ICP-MS, 700 ΜΗz NMR with cryoprobe, whole animal imaging with fluorescence and combination of ultrasound/photoacoustic, next gen sequencer and glove box systems containing thermal evaporation.
| Equipment | Short description |
| Cryo-EM and Atomic Resolution TEM, Sample preparation techniques for electron microscopy | Pretreatment of various samples. Analysis of chemical structures of biomolecules and materials at atomic level |
| Vitrobot Mark IV Systems (x2), Thermo Fisher Scientific | Semi-automated grid preparation system (vitrification of biological molecules and soft matter samples) for further study using cryo-electron microscopy |
| Talos 120 keV, Thermo Fisher Scientific | A 120 kV user-friendly transmission electron microscope for negative stain screening and evaluation that covers a diverse range of scientific imaging and experimentation needs |
| Aquilos 2+, ThermoFisher Scientific | Dedicated cryo-FIB microscope for cryo-lamellas preparation for cryo-electron tomography. Capable of producing ultra-thin, electron-transparent lamellae suitable for high-resolution cellular cryo-electron tomography or MicroED analysis of microcrystals. |
| Krios G4, ThermoFisher Scientific | High-end cryo-transmission electron microscope for molecular and cellular imaging. It supports single-particle analysis, helical filament studies, and cryo-electron tomography. Energy-filtered zero-loss imaging improves contrast and resolution, while a Falcon 4i detector and automated software enable rapid large-scale data collection. |
| Spectra 200kV, Thermo Fisher Scientific | High-performance scanning transmission electron microscope with deflection correction, designed for a wide range of applications in materials science, including 4D-STEM (four-dimensional scanning transmission electron microscopy), iDPC-STEM (integrated differential phase contrast scanning transmission electron microscopy), EELS (electron energy loss spectroscopy), EDS (energy dispersive X-ray spectroscopy) |
| SAXS/WAXS | Determination of self-assembly microstructure and structural organization of biomolecules and materials (solution, solid state, thin films) |
| Powder diffraction and upgraded protein X-ray crystallography facility
Xeuss 3.00 (Xenocs) |
Determination of structural organization of materials (solid state, thin films). Determination of the 3D structure of macromolecules and their complexes from single crystals |
| Atomic Force Microscope coupled to microRAMAN | Characterization and chemical analysis of the surface of materials in solution and thin films |
| Upgraded mouse and rat breeding facility infrastructure, equipped with in vivo bioimaging systems | Compliant with national and European standards, provides housing for mice and rats in specialized individually ventilated cages |
| Ultrasound and Photoacoustic Imaging system (Vevo F2 system (FUJIFILM VisualSonics) | Real-time monitoring of anatomical features, enabling the study of tumor development, the distribution and the activity of substances with photoacoustic properties |
| In vivo imaging φ-eye™ system (BIOEMTECH) | In vivo imaging system for small animals, enabling whole-body monitoring using fluorescence methods. Allows the study of tumor development, therapeutic responses, and the biodistribution of drugs and nanocarriers in real time. |
| Nuclear Magnetic Resonance (NMR)
Bruker Avance NEO 700 MHz with H&F/C/N TCI PRODIGY cryoprobe and refrigerated sample changer for 24 samples |
Structural analysis of small molecules, peptides and biomolecules in solution, Metabolomics, NMR-based screening assays (ligand and target based), Biomolecular NMR of Protein and Carbohydrates (structure, dynamics and interaction studies) |
| timsMetabo™, Bruker | Full omics analysis with high throughput capabilities |
| microFLEX LRF MALDI-TOF-MS, Bruker | Analysis of the molecular weights of organic compounds, polymers, and proteins |
| 7850 ICP-MS, Agilent | Analysis of metal traces and inorganic nanoparticles |
| UV-VIS-NIR / FT-IR | Electronic absorption spectroscopy of compounds and materials in the ultraviolet-visible-near infra-red region (3000 nm) |
| Steady-state and time-resolved Photoluminescence | Fluorescence emission spectroscopy of compounds and materials, in solution and solid state in the ultraviolet-visible-near infra-red region (2000 nm) |
| Next generation massive parallel sequencing
Ion GeneStudio S5 Prime System, Thermo Fisher Scientific |
Genome Sequencing capabilities and analysis |
| BioTek Cytation C10 Agilent Confocal Imaging Reader και BioTek BioSpa 8 Automated Incubator | High-throughput screening of bioactive compounds |
| High-performance computational infrastructure (816 cores, 2 GPU accelerators with 96 GB memory & 20,000 CUDA cores, system RAM: 3.5 TB, system storage: ~1 PB) | Running simulations, development of machine learning models, data analysis and storage |
| Glove boxes and thermal evaporator | Processing of materials and manufacturing of electronic devices under inert atmosphere |
| External Quantum Efficiency (EQE), Solar simulator, Litos Lite and PAIOS Advanced Electrical Characterization | Advanced spectroscopic characterization of electrical properties of electronic devices |
| Dynamic Thermomechanical analysis (DMA) | Determination of the thermomechanical properties of compounds and materials |
| High speed multiparameter Cell Sorter BD FACS ARIATM II upgrade with an additional 3rd laser (375 nm) and a liquid delivery pump in the main unit for cell isolation under sterile conditions | High Speed, 3 laser (375, 488,633 nm) Cell Sorter with capacity to achieve high sensitivity and resolution polyparametric analysis at single cell or single particle level as well as high performance cell sorting for downstream functional or genomics or transcriptomics applications |