Αναζήτηση
ΚΛΕΙΣΙΜΟΦασματοσκοπία NMR
Laboratory of Molecular Analysis
National Hellenic Research Foundation
Vas. Constantinou 48
11635, Athens, Greece
All experimental data will be treated as confidential as a matter of course. However it is also possible to arrange for a signed contract for the maintenance of confidentiality.
The NMR facility houses three spectrometers operating at 700, 600 and 300 MHz aimed at solution studies of a wide range of samples including small organic molecules, complex metabolite mixtures, peptides, proteins, polymers, and complex hydrocarbons. Both high field instruments are equipped with triple resonance cryoprobes for high sensitivity and versatility.
The facility also hosts HPC workstations, for demanding calculations, and staff with expertise in chemical, metabolomic and biological applications of NMR spectroscopy.
We provide a wide range of services in various steps of Drug discovery pipeline such as:
- small molecule structure determination
- libraries screening against pharmaceutical relevant protein targets
- hit discovery and optimization combining NMR screening with molecular modeling approaches
- protein structure, dynamics and interaction studies
Moreover, we have established mature protocols and pipelines for NMR-based metabolomics analyzing biofluids, tissue, complex mixtures of natural products with applications in
- Disease Biomarkers
- Domestic animals health promotion
- Food safety/Nutraceuticals
Instrumentation
Spectrometer shortname
V600
Specifications
- 3 RF Channel VnmrS Console/Z gradient configuration
- Variable Temperature unit (25-65 oC)
- Manual sample exchange
- Software: OpenVNMRJ 1.1
- Probe: 5mm Cryogenic triple channel HCN, cooled 1H and 13C preamplifiers (“13C enhanced”), 7700CS helium cryocooler and compressor units.
Experiments
- Complete suite of 1D and 2D experiments for small molecule/polymers structure elucidation with applications in Drug discovery and Biomaterials
- bioNMR screening for protein-ligand interaction studies (1H STD NMR, 1H-15N HSQC) supporting biomedical research
- Metabolomics pipeline supporting biomarker discovery in disease mechanisms and phenotypes, human/animal nutrition and health
- Multi-component system separation via DOSY supporting food functional products and functional nanomaterials used as nanocarriers
- Biomolecular Analysis
Multidimensional Biomolecular experiments on samples 15N/13C labelled. Full suite of experiments for assignment, structure determination and dynamics as well as protein-protein interaction
Spectrometer details for publication requirements
NMR spectra were recorded on a VARIAN VnmrS 600 MHz NMR spectrometer, equipped with a 5 mm 1H{13C,15N} cryoprobe using the software OpenVNMRJ v1.1.
Access level
Only for registered users who have undergone training. Consult Google calendar for booking time
Spectrometer shortname
V300
Specifications
- 2 RF Channel VnmrS Console/Z gradient configuration
- Variable Temperature unit (25-90oC)
- Manual sample exchange
- Software: OpenVNMRJ 1.1
- Probe: 5mm H/F/C/P Auto switchable
Access level
Walkin during daytime, consult Google calendar for overnight experiments
Experiments
1D 1H, 13C, 19F, 31P and 2D (i.e. COSY, HSQC, HMBC) spectra for routine analysis and quick screening
Spectrometer details for publication requirements
NMR spectra were recorded on a VARIAN VnmrS 300 MHz NMR spectrometer, equipped with a 5 mm H/F/C/P Auto switchable probe using the software OpenVNMRJ 1.1.
NMR facility is upgraded within the framework of the National Recovery and Resilience Plan “Greece 2.0” with a new Bruker AVANCE NEO 700 MHz spectrometer (operation in 2026).
Spectrometer shortname
Bruker700
Specifications
- AVANCE NEO console with 4 RF channels / Z-gradient operation/ fully automated acquisition capability
- Variable Temperature unit: 0°C to +150°C
- Refrigerated auto sample changer (24 position)
- Software: TopSpin4
- Probe: Prodigy TCI cryoprobe (N2 cooled) H&F/C/N 5mm
1H optimized 5mm triple resonance TCI H&F; 1H or 19F observation; 13C observation with 1H or 19F decoupling with cooled preamplifiers for 1H & 19F, 13C and 2H.
Access level
Only for registered users who have undergone training.
Experiments
- Complete suite of 1D and 2D experiments for small molecule/polymers structure elucidation with applications in Drug discovery pipeline and Biomaterials
- bioNMR screening for protein-ligand interaction studies (1H STD NMR, 1H-15N HSQC) supporting biomedical research
- Metabolomics pipeline supporting biomarker discovery in disease mechanisms and phenotypes, human/animal nutrition and health
- Multi-component system separation via DOSY supporting food functional products and functional nanomaterials used as nanocarriers
- Biomolecular Analysis
Multidimensional Biomolecular experiments on samples 15N/13C labelled. Full suite of experiments for assignment, structure determination and dynamics as well as protein-protein interaction
Laboratory of Molecular Analysis
National Hellenic Research Foundation
Vas. Constantinou 48
11635, Athens, Greece
All experimental data will be treated as confidential as a matter of course. However it is also possible to arrange for a signed contract for the maintenance of confidentiality.