Curriculum Vitae

THOMAS MAVROMOUSTAKOS
Chemist, Research Associate

Date of birth: 18/6/1960
Address: National Hellenic Research Foundation, Vas. Constantinou 48, 116 35 Athens
Tel: (301) 7273869
FAX: (301) 7272872
e-mail: tmavro
Family status: Married with four children

EDUCATION

B.Sc. 1985 Chemistry, University of Athens, Greece.

M.Sc. 1988 Medicinal Chemistry, University of Connecticut

Ph.D. 1990, Medicinal Chemistry, University of Connecticut.

EMPLOYMENT

1985-1987. Teaching Assistant in School of Pharmacy for introductory and advanced level courses.

1988-October 1990. Research Assistant in School of Pharmacy.

October 1990-June 1991 Postdoctoral Fellow in School of Pharmacy.

June 1991-September 2004 Researcher director at Laboratory of Molecular Analysis (LMA) at the Institute of Organic and Pharmaceutical Chemistry (IOPC) of National Hellenic Research Foundation (NHRF).

September 2004-January 2005 Visiting associate professor in the University of Cyprus (Chemistry Department). Teaching of Organic Chemistry laboratory and Medicinal Chemistry.

January 2005- Director of Laboratory of Molecular Analysis (LMA) at IOPC.

Brief Description of My Research Activities

• My research activities focus in the design of novel drugs against hypertension.

To achieve this aim I use the following physical chemical methodologies:
a. Solid State Nuclear Magnetic Resonance in order to study the dynamic changes it causes the antihypertensive drug in the lipid bilayers. In addition the orientation of drugs in lipid bilayers is sought using deuterium NMR in collaboration with synthetic chemists who deuterate specific sites of drugs.
b. High Resolution Nuclear Magnetic Resonance in duterated solvents or environments that simulate the active site of action.
c. Conformational Analysis Using NMR ROE data in combination with computational analysis and molecular modeling to complement the experimental results.
d. Differential Scanning Calorimetry to study the thermal changes that causes the presence of pharmaceutical compounds with model membranes.
e. X-ray diffraction where the topography of drugs is sought in lipid bilayers. The X-ray diffraction experiments are run in the Biophysical Institute of Graz (Austria) under the guidance of Prof. P. Laggner.
f. Raman Spectroscopy to study the interactions of bioactive compounds with lipid bilayers. In particular, the trans:gauche ratio is estimated at the different mesomorphic states of lipid bilayers. The experiments are performed in the Chemistry Department of Athens under the guidance of Prof. K. Viras.
g. 3D-QSAR studies. CoMFA and CoMSIA softwares are applied to reveal the stereoelectronic requirements for a drug to exert biological activity. 3D-QSAR studies is a valuable tool in the rational design.
h. Simulation of NMR spectra in liquid and solid state with the collaboration of theoretical physical chemists.
k. Docking studies of antihypertensive drugs with AT1 receptor.

A part of my research activity is dedicated in the authenticity aspects of beverages and foods.
a. Study of geographical origin and adulteration of virgin olive oil by other cheaper oils using high resolution 13C-NMR of olefinic region. In addition it is studied the adulteration of refined oil by refined hazelnut oil.
b. Study of authenticity of Cypriot beverage zivania with the combination of different physical chemical methods in collaboration with the University of Cyprus ( 1Η-NMR and Raman Spectroscopies, ICP, Gas Chromatography, Refractometry, Statistical Analysis to facilitate the analysis of the output data etc).

• Provide services and solve research problems

Use the modern high technology infrastructure of the institute to collaborate and solve some of the research problems facing by professors at Greek Universities, Technical Schools or Private Units (i.e. Pharmaceutical Companies). Such problems include structure determination of zeolithes, inorganic complexes and soil materials, polymorphism of pharmaceutical compounds. The income of these services are used to subsidise students and repair of laboratory equipment.


LIST OF PUBLICATIONS 2004-2005
1. A.N. Batsoulis, M.K. Nacos, C.S. Pappas, P.A. Tarantilis, T. Mavromoustakos, M.G. Polissiou. Determination of uronic acids in isolated hemicelluloses from kenaf using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and curve-fitting deconvolution method. Applied Spectroscopy 58(2) 199-202 (2004).

2. I. Kyrikou, I. Daliani, T. Mavromoustakos, H. Maswadeh, C., Demetzos, S Xatziantoniou, S. Giatrellis, G. Nounesis. The modulation of thermal and dynamic properties of vinblastine by cholesterol in membrane bilayers BBA M BIO. 1661, 1-8 (2004).

3. K. Alexopoulos, P. Fatseas, E. Melissari, D. Vlahakos, P. Roumelioti, T. Mavromoustakos, S. Mihailescu, M. Christina, P. Carbajal, D. Mascher, J. Matsoukas. Design and Synthesis of Novel Biologically Active Thrombin Receptor Non Peptide Mimetics Based on the Pharmacophoric Cluster Phe/Arg/NH2 of the Ser42-Phe-Leu-Arg46 Motif Sequence: Platelet Aggregation and Relaxant Activities J. Med. Chem. 47(13), 3338-3352 (2004).

4. K.S. Triantafyllidis, L. Nalbandian, P.N. Trikalitis, A.K. Ladanos, T. Mavromoustakos, C.P. Nicolaides. Structural, compositional and acidic characteristics of nanosized amorphous or partially crystalline ZSM-5 zeolite-based materials. Microporous and Mesoporous Materials 75, 89-100 (2004).

5. D. Kovala-Demertzi, C. Vidjeluc, M.A. Demertzis, E. Siapi, T. Mavromoustakos.
The thermal effects of platinum (II) and palladium(II) complexes with 2-acetyl pyridine and pyridine-2-carbaldehyde N(4)-ethyl-thiosemicarbazones in membrane bilayers. Thermochimica Acta, 42(1-2), 53-58 (2004)

6. I. Kyrikou, S. Xadjikakou, D. Kovala-Demertzi, K.Viras, T. Mavromoustakos, Effects of non steroid anti-inflammatory drugs in membrane bilayers containing cholesterol. Chem. Phys. Lipids 132, 157-169 (2004).

8. T. Mavromoustakos, P. Zoumpoulakis, I. Kyrikou, A. Zoga, E. Siapi, M. Zervou, I. Daliani, D. Dimitriou, A. Pitsas, C. Kamoutsis, P. Laggner.Efforts to Understand the Molecular Basis of Hypertension Trough Drug:Membrane Interactions. Current Topics in Medicinal Chemistry, 4, 445-459 (2004).

9. T. Mavromoustakos, M. Zervou, P. Zoumpoulakis, I. Kyrikou, N.P. Benetis, L. Polevaya, P. Roumelioti, N. Giatas, A. Zoga, P. Moutevelis Minakakis, A. Kolocouris, D. Vlahakos, S. Golic Grdadolnik, J. Matsoukas. Conformation and Bioactivity. Design and Discovery of Novel Antihypertensive Drugs. Current Topics in Medicinal Chemistry, 4, 385-401, (2004).
10. K.P. Marathias, B. Agroyannis, T. Mavromoustakos, J. Matsoukas,D.V. Vlahakos. Hematocrit-lowering Effect Following Inactivation of Renin-Angiotensin System with Angiotensin Converting Enzyme Inhibitors and Angiotensin Receptor Blockers. Current Topics in Medicinal Chemistry, 4, 483-486 (2004).

11. Conformational Analysis Using 2D NMR Spectroscopy Coupled with Computational Analysis as an Aid in the Alignment Procedure of 3D-QSAR studies. T. Mavromoustakos, A. Kapou, N.P. Benetis, M. Zervou. Drug Design Reviews-Online 1(3) 235-245 (2004).

12. I. Kyrikou, T. Mavromoustakos, S. Xatziantoniou, A. Georgopoulos, C. Demetzos. A comparative study of the effects of cholesterol and sclareol, a bioactive labdane diterpene, on phospholipids bilayers Chemistry and Physics of Lipids 133, 125-134 (2005).

13. The Use of Differential Scanning Calorimetry to Study the Effects of Gentamycin on Fibrous Collageneous Membranes. E. Siapi, T. Mavromoustakos, V.Trandafir , B. Albu and P. Budrugeac. Thermochimica Acta 425, 165-171 (2005).

14. J. Matsoukas, V. Apostolopoulos, H. Kallbacher, A.M. Papini, T. Tselios, K. Chatzantoni, T. Biagioli, F. Lolli, S. Deraos, P. Papathanassopoulos, T. Mavromoustakos, A. Mouzaki. Enhanced Stability and Biological Properties of Myelin Basic Protein Epitope 87-99 Cyclic Mimics Render Them a Potentially New Class of Immunomodulators J. Med. Chem. 48,1470-1480 (2005).

15. A. Kapou, T. Mavromoustakos, S. Nikolaropoulos, Thermal Effects of Steroidal Carriers of Alkylating Agents On DPPC Membrane Bilayers. Thermochimica Acta 429, 53-56 (2005).

16. E.T. Kefalas, P. Panagiotidis, C.P. Raptopoulou, A. Terzis, T. Mavromoustakos, A. Salifoglou. Mononuclear Titanium(IV)-Citrate Complexes from Aqueous Solutions:pH-Specific Synthesis and Structural and Spectroscopic Studies in Relevance to Aqueous Titanium(IV)-Citrate Speciation. Inorg. Chem. 44, 2596-2605 (2005).

17. Nikolas-P. Benetis, Ioanna Kyrikou, Thomas Mavromoustakos, Maria Zervou. Static 31P CP NMR Multilamellar Bilayer Broadlines in the Absence and presence of the Bioactive Dipeptide beta-Ala-Tyr and Comparison with Glu" Chemical Physics 314(1-3) 57-72 (2005). .

18. P. Petrakis, I. Touris, M. Liouni, M. Zervou, C.R. Kokkinofta, R. Theocharis, T. Mavromoustakos. Authenticity of the traditional Cyprus spirit "Zivania" on the basis of 1H NMR Spectroscopy Diagnostic Parameters and Statistical Analysis. P. Petrakis, I. Touris, M. Liouni, M. Zervou, C.R. Kokkinofta, R. Theocharis, T. Mavromoustakos (in press J. Agric. Food Chem.).

19. A. Kapou, M.A. Fousteris, S.S. Nikolaropoulos, M. Zervou, S.G. Gardadolnik, P. Zoumpoulakis, I. Kyrikou, T. Mavromoustakos. 2D NMR and conformational analysis of a prototype anti-tumor steroidal ester. (in press J. Pharmaceut. Biomed.).

20. E.T. Kefalas, M. Dakanali, P. Panagiotidis, C.P. Raptopoulou, A. Terzis, T. Mavromoustakos, I. Kyrikou, N. Karligiano, A. Bino, A. Salifoglou. pH-Specific Aqueous Synthetic Chemistry in the Binary Cadmium(II)-Citrate System. Gaining Insight into Cadmium(II)-Citrate Speciation with Relevance to Cadmium Toxicity. Inorg. Chem. (in press).

21. E.D. Manzourani, T.M. Mavromoustakos, J.A. Platts, J.M. Matsoukas, T.V. Tselios. Structural Requirements for Binding of Myelin Basic Protein (MBP) Peptides to MHC II: Effects on Immune Regulation. Current Medicinal Chemistry, 12, 763-771 (2005).