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Marie Curie Actions TOK-Development Project MTKD-CT-2004-509836 (MACROGENEX)


Objectives

The scientific and technological goals achieved so far in the context of the project are:

Scientific hightlights:
Task A. ''TFIID-AP 1 co-operation''
-Functional evaluation of the TFIID-AP 1 interaction
- Target gene specific TFIID-AP 1 co-operation at the chromatin level.

Task B. ''Nuclear receptor regulation of cell proliferation and differentiation''
- Role of TAC and retinoid receptors in embryonal (P19) cell differentiation.
- Effects of retinoids on the proliferation and survival of breast cancer cells.
- Glucocorticoid receptor transcriptional targets on a genome-wide scale.

Task C. ''Novel multi-protein complexes of the transcription factor TAF15''
-Association of TAF15 with a subset of the spliceosomal U1-snRNP complex
-Domain properties related to cellular interactions of TAF15
-Interaction of hnRNP M -an additional TAF15 associated component- with factors involved in transcription and RNA processing

Technological highlights:
Assimilation of new technologies:
ChIP technology
Genome-wide probing of nuclear receptor target genes by ChIP-on-chip/sequencing
RNAi technology
Microarray technology

Development of new tools:
New expression vectors and reporter genes
New reporter cell lines
Bioinformatic tools
New synthetic or plant-derived nuclear receptor ligands
Production of ChIP-grade antibodies (now marketed: http://www.diagenode.com/)

 

 

 

 

 

 
 

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